Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pruzinska, A.; Tanner, G.; Anders, I.; Roca, M.; Hortensteiner, S.
    Chlorophyll breakdown: pheophorbide a oxygenase is a Rieske-type iron-sulfur protein, encoded by the accelerated cell death 1 gene (2003), Proc. Natl. Acad. Sci. USA, 100, 15259-15264.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.17 expression in Escherichia coli Arabidopsis thaliana
1.14.15.17 gene PaO, functional recombinant expression in Escherichia coli strain C43 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.17 additional information
-
 additional information Michaelis-Menten kinetics Arabidopsis thaliana
1.14.15.17 0.006
-
 pheophorbide a pH 7.0, 24°C, recombinant enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.17 chloroplast envelope PaO is localized in the chloroplast inner envelope Arabidopsis thaliana 9941
-
1.14.15.17 gerontoplast the enzyme contains a chloroplast transit peptide Arabidopsis thaliana 34400
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.17 Fe Rieske-type iron-sulfur cluster-containing protein Arabidopsis thaliana
1.14.15.17 Iron-sulfur cluster AtPaO is a Rieske-type iron–sulfur cluster-containing enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.17 additional information Arabidopsis thaliana PaO expression is correlated positively with senescence. The in vivo function of PaO is the degradation of pheide a during senescence ?
-
 ?
1.14.15.17 pheophorbide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Arabidopsis thaliana
-
 red chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.17 Arabidopsis thaliana
-
-
-
1.14.15.17 Arabidopsis thaliana Q9FYC2 gene PaO
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.17 leaf
-
 Arabidopsis thaliana
-
1.14.15.17 additional information PaO activity is found only during senescence Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.17 additional information PaO expression is correlated positively with senescence. The in vivo function of PaO is the degradation of pheide a during senescence Arabidopsis thaliana ?
-
 ?
1.14.15.17 pheophorbide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Arabidopsis thaliana red chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.17 ACD1
-
 Arabidopsis thaliana
1.14.15.17 AtPaO
-
 Arabidopsis thaliana
1.14.15.17 PAO
-
 Arabidopsis thaliana
1.14.15.17 pheophorbide a oxygenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.17 24
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.17 7
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.17 Ferredoxin
-
 Arabidopsis thaliana
1.14.15.17 NADPH
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.15.17 evolution AtPaO is a Rieske-type iron–sulfur cluster-containing enzyme that is identical to Arabidopsis accelerated cell death 1 and homologous to lethal leaf spot 1 (LLS1) of maize Arabidopsis thaliana
1.14.15.17 metabolism enzyme PaO seems to be a key regulator of chlorophyll catabolism Arabidopsis thaliana
1.14.15.17 physiological function chlorophyll breakdown during senescence is an integral part of plant development and leads to the accumulation of colorless catabolites. The loss of green pigment is due to an oxygenolytic opening of the porphyrin macrocycle of pheophorbide (pheide) a followed by a reduction to yield a fluorescent chlorophyll catabolite. This step is comprised of the interaction of two enzymes, pheide a oxygenase (PaO) and red chl catabolite reductase. Senescence-related activity of PaO Arabidopsis thaliana